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Analysing microbial communities from RestREco project

Differential abundance (ANCOMBC2)

Differential abundance analysis using ANCOMBC was carried out on unrarefied data due to the recommendations by the package authors and discussions online. For significant taxa associated with different site-treatment groups, the data was also non-aggregated to ensure there were enough samples for ANCOM analysis.

Bacteria

Genera

Treatment

No genera were identified as differentially abundant when comparing control and water stress samples

## [1] "Control" "Drought"
Comparing control and shelter samples on each site

No differentially abundant taxa were identified between control and water stress samples on any of the sites

Comparing control samples between sites
Comparing shelter samples between sites

Family - control vs rain shelter on each site

No differentially abundant families were identified between control and water stress samples on any site

Phylum

Treatment

No phyla were identified as differentially abundant when comparing control and water stress samples

Comparing control and shelter samples on each site

No differentially abundant phyla were identified as responding to changes in soil moisture on any site

Comparing control samples between sites
Comparing shelter samples between sites

Fungi

Genera

Treatment

No differentially abundant genera were identified between control and water stressed samples

Comparing control and shelter samples for each site
Comparing control samples between sites
Comparing shelter samples between sites

Family - control vs rain shelter on individual sites

Phylum

Treatment
Comparing control and shelter samples on each site

No differentially abundant phyla were identified as responding to changes in soil moisture on any site

Comparing control samples between sites
Comparing shelter samples between sites